A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.
Get tips on using FastLane Cell cDNA Kit (50) to perform cDNA synthesis Cell lines
Get tips on using iScript cDNA Synthesis Kit to perform
Get tips on using iScript cDNA Synthesis Kit to perform
Get tips on using iScript cDNA Synthesis Kit to perform AAA for reviews
Get tips on using Transcriptor First Strand cDNA Synthesis Kit to perform cDNA synthesis Yeast
Get tips on using Transcriptor High Fidelity cDNA Synthesis Kit to perform cDNA synthesis Tissue
Get tips on using Transcriptor First Strand cDNA Synthesis Kit to perform cDNA synthesis Bacteria
Get tips on using RevertAid First Strand cDNA Synthesis Kit to perform cDNA synthesis Bacteria
Get tips on using High-capacity cDNA reverse transcription kit to perform cDNA synthesis Cell lines
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment